Curr Protoc Immunol. 2001 May;Chapter 20:Unit 20.2. doi: 10.1002/0471142735.im2002s39. Mouse 4T1t tumor model. Pulaski BA 1 , Ostrand-Rosenberg .When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes andin while the .The 4T1 mouse mammary tumor cell line is one of only a fewt cancer models with the capacity to metastasize efficiently to sites affected in humant .As a control, we produced a stable triple-positivet cancer TPBC cell line by transfecting 4T1 cells with rat HER2, ER and PgR genes and sorted for cells .
Curr Protoc Immunol. 2001 May;Chapter 20:Unit 20.2. doi: 10.1002/0471142735. im2002s39. Mouse 4T1t tumor model. Pulaski BA 1 , Ostrand-Rosenberg .The 4T1 mouse mammary tumor cell line is one of only a fewt cancer models with the capacity to metastasize efficiently to sites affected in human t .As a control, we produced a stable triple-positivet cancer TPBC cell line by transfecting 4T1 cells with rat HER2, ER and PgR genes and sorted for cells . I 'm growing 4T1 cells in mice. I 'm implanting two contralateral tumors per mouse. I 'm having a hard time getting the tumors to grow .
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